Perceptual integration of tertiary taste mixtures

Integration psychophysics was used to explore the taste perception of mixtures of sucrose, fructose, and citric acid. Three levels of each stimulus were varied in a 3 x 3 x 3 factorial design. Subjects rated total intensity, sweetness, and acidity of the 27 mixtures on graphic rating scales. Consistent with earlier work, the perceived total intensity of the tertiary mixtures was found to be dictated by the intensity of the (subjectively) stronger component alone (i.e., either the integrated sweetness or the acidity, whichever was the more intense). In contrast, the sweetness and acidity of the mixture were susceptible to mutual suppression: Sweetness suppressed acidity, acidity suppressed sweetness. There was, however, a difference between sucrose and fructose in their interactions with citric acid, fructose being the more susceptible to suppression. This selectivity of suppression indicates that the two sweetnesses could not have been inextricably integrated. Implications for taste coding are discussed, and the findings are reconciled in terms of two separate coding mechanisms: one for taste intensity, another for taste quality.     

Qualitative identification of the taste of weak sucrose-sodium chloride mixture stimuli

Near-threshold mixtures of sucrose and sodium chloride were shown to elicit acidic or bitter sensations as well as, or instead of, sweetness and saltiness. The probability of a bitter response was relatively stable and consistent with error due to residual noise in the identification task. The acidic response varied in probability with the sodium chloride concentration, and was generally substitutive for a sweet response.     

The effectiveness of different sweeteners in suppressing citric acid sourness

The exact mechanism that causes taste suppression in a perceptually heterogeneous mixture, and the locus of that mechanism, are as yet unknown. The present study was designed to explore the idea that mixture suppression is a perceptual phenomenon and not the result of physical, chemical, or receptor-substance interactions. An investigation was carried out as to whether perceptually similar taste stimuli give rise to the same sensory interactions when mixed with a substance of a different taste quality. In the first study, five different sweeteners (sucrose, fructose, aspartame, saccharin, and sorbitol) were matched in perceived sweetness intensity, in order to obtain five perceptually similar stimuli. Every equisweet sweetener concentration was mixed with each of four citric acid concentrations. In a second study, the sourness-suppressing effects of two sweeteners, sucrose and aspartame, were compared at four different concentration levels. Sourness scale values of unmixed citric acid, the unmixed sweeteners, and the citric acid/sweetener mixtures were assessed with a functional measurement approach in combination with a two-stimulus procedure. The equisweet sweeteners were equally effective in suppressing the perceived sourness intensity of citric acid over the concentration range used. The side tastes of the sweeteners, if present, did not have a substantial effect on the degree of sourness suppression.     

Hedonic and sensory characteristics of odors conditioned by pairing with tastants in humans

Animals readily acquire positive odor-taste hedonic associations, but evidence for this in humans remains weak and was explored further. Retronasal pairing of odors with sucrose or salty stimuli (Experiment 1) increased the rated sweetness of sucrose-paired odors without altering liking, although changes in odor pleasantness correlated with sucrose liking. Experience of odors with sucrose or quinine by sweet likers (Experiment 2) found increased pleasantness and sweetness for sucrose-paired odors, whereas quinine-paired odors became less liked and more bitter. Odor-sucrose pairings in sweet likers and dislikers (Experiment 3) found increased sweetness in both groups but increased odor liking only in likers. These data suggest that evaluative and sensory learning are dissociable and that evaluative changes are sensitive to individual differences in sweet liking.     

Sweetness and intensity of artificial sweeteners

In four experiments, groups of Os judged either the sweetness or the entire taste intensity of solutions of sucrose, cyclamate salts, cyclamate-saccharin mixtures, and sodium saccharin. The sensory functions obtained by magnitude estimation suggest that over the middle range of concentration the sweetness and intensity of the foregoing substances grow as power functions of concentration. As a first approximation, the exponents for sweetness and intensity are, respectively, 1.6 to 1.4 for sucrose, 1.0 to 0.8 for cyclamate salts, 0.6 to 0.85 for cyclamate-saccharin mixtures, and 0.3 to 0.6 for sodium saccharin.     

Quality-specific effects of aging on the human taste system

Elderly persons are known to have elevated taste thresholds, with those for bitter more affected by age, for example, than those for sweet. Do analogous quality-specific effects occur at suprathreshold levels? Young (mean age = 20.3 years, SD = 2.99) and elderly (mean age = 72.5 years, SD = 4.58) subjects made magnitude estimates of sweetness, bitterness, sourness, and saltiness for the unmixed components sucrose, caffeine, citric acid, and NaCl at three concentration levels for each. They also made magnitude estimates of the separate taste qualities in two-component mixtures of sucrose with each of the other three qualities, at various levels of the two components in each mixture. Magnitude estimates of taste intensity were interweaved with magnitude estimates of the heaviness of six weights, which subjects were to judge on the same subjective intensity scale: This is the calibration feature of the method of magnitude matching, and permits the comparison of elderly and young subjects on the absolute intensity of tastes. When unmixed components were judged, elderly subjects found the characteristic tastes of caffeine and citric acid less intense than, but those of sucrose and NaCl as intense as, younger subjects did. In judging mixtures, the elderly found bitterness, but not the other three qualities, less intense than did the young subjects.     

Gustatory cross adaptation between sweet-tasting compounds

Taste profiles were obtained for 16 compounds after adaptation to sucrose, saccharin, and water. Sucrose adaptation reduced the sweetness of all sweet compounds. Saccharin adaptation, when analyzed over all compounds, also reduced sweetness, but the effect was less than that of sucrose. It is concluded that there may be a single receptor mechanism for the sweet quality. Adaptation to sucrose also increased the saltiness, sourness, and bitterness of the other compounds slightly. This increase should be attributed to the taste : induced in water by adaptation to sucrose rather than a potentiation of the other compounds per se.     

Sweet odours and sweet tastes are conflated in memory

Certain odours and certain tastes appear to share common perceptual properties. One example is sweetness, a perceptual experience that results from stimulation of taste receptors on the tongue typically by sugars. The experiment here examined for evidence of this perceptual similarity using a novel and indirect test. Participants were exposed six times each, to three odours (strawberry, caramel, and oregano) and three tastes (sucrose, saline, and citric acid). Following a 10-min interval, participants were given a surprise frequency estimation task, in which they had to judge how often each stimulus had occurred. If sweet-smelling strawberry and caramel odours really do share this perceptual characteristic in common with sweet tasting sucrose, then frequency estimates for sucrose should be overestimated relative to non-sweet tastes. Not only was this observed, but frequency estimates for sweet tastes were also found to correlate with (1) evaluations from a later test of similarity between these sweet smells and sucrose, and (2) the degree to which these odours smelled sweet. These findings suggest a shared perceptual feature between such odours and sucrose - sweetness - under conditions where no judgment of perceptual quality was required.     

Acquired hedonic and sensory characteristics of odours: Influence of sweet liker and propylthiouracil taster status

Repeated pairings of novel food-related odours with sweet tastes can result in enduring changes in sweetness of the odour alone, but have less consistent effects on odour liking. Variation in ability to taste propylthiouracil (PROP) might account for this, since PROP supertasters (ST) have been reported both to experience stronger sweetness intensity and to be more likely to dislike sweetness than do PROP nontasters (NT). Alternatively, individual differences in liking for sweetness may transfer to sweet-paired odours independently of PROP sensitivity. To explore this, evaluations of sucrose, saccharin, and PROP solutions were used to classify 92 volunteers as either sweet likers or dislikers and as PROP ST, NT, or medium tasters (MT). Changes in pleasantness of odours that had been paired with the taste of saccharin increased in sweet likers but decreased in dislikers. Odour sweetness increased regardless of PROP taster or sweet liker status. PROP ST rated saccharin as more bitter than did other taster groups and also showed greater increases in acquired bitterness of the saccharin-paired odour. Overall, these data suggest that individual differences in evaluation of saccharin reliably predict subsequent changes in evaluation of saccharin-paired odours, with hedonic changes corresponding with liking for sweet tastes and sensory changes reflecting differences in sensory quality between PROP taster groups.     

Adapting efficiency of salt-sucrose mixtures

Previous studies had observed that NaCI-sucrose mixtures cross-adapted solutions of their unmixed components just as effectively as adaptation to the components themselves, while quinine-sucrose mixtures were not as effective in cross-adapting their components. The present study investigated whether these effects were due to the different tastants involved or to different conditions of stimulation and adaptation employed. Adaptation to NaCI-sucrose mixtures using the conditions of the quinine-sucrose study resulted in perfect cross-adaptation of NaCI saltiness by the mixture, but only partial adaptation of sucrose sweetness by the mixture. A second study attempted to reconcile this remaining difference by examining the stimulation parameters of flow rate, temperature, and area stimulated. As flow rate and area were decreased, the difference between mixture adaptation and self-adaptation of sucrose was attenuated. Mixture suppression may depend upon the tastants mixed and the parameters of stimulation, and may be the result of several physiological mechanisms.     

Human taste contrast and self-reported measures of anxiety.

Successive negative taste contrast in humans was demonstrated with a common taste stimulus, i.e., cherry-flavored Kool-Aid. A total of 31 male and female college-aged participants rated a 7% sucrose solution which was cherry-flavored as less sweet when it was preceded by a 28% rather than a 7% sucrose solution which was cherry-flavored. Because drugs such as the benzodiazepines affect taste contrast in rats and act as anxiolytics in humans, the present experiment also examined whether several self-reported measures of anxiety were related to taste contrast in humans. Neither scores on Taylor's Manifest Anxiety Survey nor those on the State-Trait Anxiety Inventory were related to "sweetness" ratings or contrast effects.     

Both perceptual and conceptual factors influence taste-odor and taste-taste interactions

Observers are often asked to make intensity judgments for a sensory attribute of a stimulus that is embedded in a background of “irrelevant” stimulusdimensions. Under some circumstances, these background dimensions of the stimulus can influence intensity judgments for the target attribute. For example, judgments of sweetness can be influenced by the other taste or-odor qualities of a solution (Frank & Byram, 1988; Kamen et al., 1961). Experiments 1 and 2 assessed the influence of stimulus context, instructional set, and reference stimuli on cross-quality interactions in mixtures of chemosensory stimuli. Experiment 1 demonstrated that odor-induced changes in sweetness judgments were dramatically influenced when subjects rated multiple attributes of the stimulus as compared with when they judged sweetness alone. Several odorants enhanced sweetness when sweetness alone was judged, while sweetness was suppressed for these same stimuli when total-intensity ratings were broken down into ratings for the sweetness, saltiness, sourness, bitterness, and fruitiness of each solution. Experiment 2 demonstrated a similar pattern of results when bitterness was the target taste. In addition, Experiment 2 showed that the instructional effects applied to both taste-odorand taste-taste mixtures. It was concluded that the taste enhancement and suppression observed for taste-odor and taste-taste mixtures are influenced by (1) instructional sets which influence subjects’ concepts of attribute categories, and (2) the perceptual similarities among the quality dimensions of the stimulus.     

Perceptual changes in taste mixtures

Three studies were conducted to quantify perceptual changes that occur when sapid chemicals are tasted in mixture solutions. The primary effect when mixing sweetness (glucose or fructose) with salt (NaCl), sour (citric acid), or bitter (quinine sulfate) was to reduce the intensity of each taste in the mixture. The reduction was not equal for the two components, although the overall (total) taste intensity of the mixture appeared to be approximately 50% of the sum of the intensities of the unmixed components. Mixtures of sweet and salt developed an “unblended” or “clashing” taste, in which the components alternated in attempting to dominate the taste percept. Sweet mixed with either sour or bitter blended in almost all proportions. The “flavor” of sweetness in mixtures differed from that of simple sugar sweetness, suggesting that the presence of a second taste modified the qualitative aspect of sweetness. The magnitude of change in sweetness quality depended upon the sugar being rated, and upon the quality and intensity of the second, or modifying, taste.     

Effects of context in judgments of sweetness and pleasantness

Dramatic effects of the immediate stimulus context were demonstrated for ratings of sweetness and also for ratings of pleasantness of soft drinks containing different concentrations of sucrose. The same drinks were rated sweeter when the lower concentrations were presented more frequently, less sweet when the higher concentrations were presented more frequently. A quasi-normal distribution of frequencies yielded ratings falling between the two skewed distributions. Ratings of sweetness were accurately predicted by Parducci’s (1974) range-frequency model of judgment, which was originally developed to explain contextual effects in other psychophysical dimensions. Ratings of pleasantness were also affected by context; the highest ratings were assigned to concentrations of intermediate sweetness in their respective contexts.     

Effects of solution viscosity on perceived saltiness and sweetness

Subjects used magnitude estimation to judge the perceived saltiness or sweetness of a series of aqueous solutions containing five suprathreshold concentrations of NaCl or sucrose and thickened with sodium carboxymethylcellulose (CMC). In the first experiment, CMC-H (high viscosity form) was used to thicken a series of sucrose and NaCl solutions to six viscosity levels (1–2,025 centistokes). At the highest viscosity levels, significant decreases occurred in the perceived taste intensity of only the lower concentrations of sucrose and NaCl. A second experiment determined that variations in the quantity of solution sampled from cups did not systematically influence judgments of saltiness when the starting volume was 10 ml. In the third experiment, aqueous solutions containing sucrose or NaCl were thickened with the low (L), medium (M), or high (H) viscosity form of CMC (1–1,296 centistokes). CMC-L-thickened solutions produced little or no suppression of perceived taste intensity, whereas viscous CMC-H solutions produced significant reductions in perceived saltiness and sweetness.     

Assessment of the taste interaction between two qualitatively similar-tasting substances: a comparison between comparison rules

The taste interaction between sucrose and fructose was assessed by using three different comparison procedures: the summated response comparison, the factorial plot comparison, and the equimolar comparison rule. The perceived sweetness intensities were obtained on a ratio scale by using a functional measurement approach in combination with a two-stimulus procedure. The conclusions obtained from each of the three comparison rules were identical. The taste interaction between sucrose and fructose could be explained to a large extent, but not completely, by the apparent taste "interactions" within sucrose and fructose as single substances. It is argued that the apparent taste interaction within a large number of single sugars and between two of these sugars in a mixture is somewhat synergistic at low sweetness levels, additive at intermediate sweetness levels, and suppressive at high sweetness levels.     

Sweetness suppression in fructose/citric acid mixtures: A study of contextual effects

Twoexperiments investigated whether stimulus context affects ratings for mixtures of dissimilartasting substances (fructose/citric acid) to the same degree that it affects ratings for unmixed substances (fructose). In Experiment 1, replacing mixtures by equisweet unmixed fructose solutions produced virtually no response shifts. The proportion of mixtures in the stimulus set affected only slightly the degree of mixture suppression inferred from the responses. In Experiment 2, both the stimulus type (mixed or unmixed) and the stimulus distribution (positively versus negatively skewed) affected the responses. Several factors that determine the impact of contextual changes are identified: (a) the stage in stimulus processing affected—that of representation on the internal continuum or that of response selection; (b) the size and sources of variation in the affected process; and (c) the degree to which a stimulus is perceptually integrated in the context. In the present study, the sweetness of fructose/citric acid mixtures was largely, but not completely, integrated with the sweetness of unmixed fructose solutions. It is suggested that increased stimulus complexity makes mixture ratings more susceptible to contextual shifts. An analysis relating the size of the contextual shift to the degree of response variability suggests that response-selection processes are more important in determining the responses for unmixed stimuli than they are in determining the responses for mixtures.     

How Sweetness Plays Sweetly in Persuasion

Three experiments explored how sweetness metaphors affect individuals’ attitudes. The results indicated that sweetness metaphors led to positive responses to the target advertisement and the advertised product, both when the sweetness was actually experienced (Experiment 1) and when the sweetness experience was imagined (Experiment 2 and 3). In Experiment 1, participants who directly experienced a sweet taste evaluated a mineral water advertisement more positively than those who did not experience a sweet taste. Experiment 2 showed that an imagined sweet taste generated favorable attitudes toward the same targets as in Experiment 1. Results of Experiment 3, which used advertisements of various product categories, were similar to those of the previous two experiments, where both direct experience and mental imagination of sweetness were advantageous for forming favorable attitudes.     

Taste-aroma interactions in a ternary system: a model of fruitiness perception in sucrose/acid solutions

Cross-modal interactions between aroma, sweetness, and acidity were studied. A series of samples was presented to trained panelists who assessed strawberry flavor intensity using magnitude estimation with a reference modulus. The delivery of aroma stimuli from the different solutions was measured by monitoring exhaled breath using atmospheric pressure chemical ionization-mass spectrometry to determine whether there were any physicochemical effects on volatile release; no significant differences were noted. Three-dimensional predictive models were built to describe perceived strawberry flavor intensity as a function of concentrations of sucrose, acid, and volatiles. Analysis of the data identified two groups of panelists with different responses: For Group 1, increasing sucrose and/or acid levels also increased the perceived flavor intensity. For Group 2, changing sucrose concentrations had little effect, but increasing acid and/or volatile levels did. The results show different effects of organic and inorganic acids on perception, as well as clear interactions between the modalities of taste (sugar and acid) and aroma. The clustering of panelists' responses suggests that this phenomenon may depend on prior associations between the fruity flavor and the tastants.     

Ratio scales of sugar sweetness

In a series of 10 experiments, groups of Os judged the sweetness of 16 sugars. The results suggest that, for all sugars except mannose, the intensity of sweetness grows as a power function of concentration, with an exponent of about 1.3. The relative sweetness of sugars was determined using both molarity and per cent by weight. With both measures, sucrose and fructose were the sweetest sugars. The order of the remaining sugars in the sweetness hierarchy was partiy a function of the measure of concentration. The variability of the magnitude estimates of sweetness was roughly proportional to the stimulus concentration, supporting Weber’s law.