Sensation seeking and stimulus intensity as modulators of cortical,cardiovascular, and electrodermal response: A cross-modality study

Previous studies have related the trait of sensation seeking to augmenting of the evoked potential (EP) in both visual and auditory modalities and to electrodermal and heart rate (HR) orienting and defensive reactions. The present study examined all of these phenomena in the same sample of subjects in order to replicate previous findings, and investigate cross-modality consistency and relationships between cortical and peripheral responses. Fifty-four male subjects, scoring high or low on the Disinhibition Sensation Seeking Scale, were exposed to 4 intensities of auditory stimuli (tones) on one occasion and visual stimuli (light flash) on another. Two interstimulus (ISIs) intervals were used for each set of stimuli: first a 17 sec series, and then a 2 sec series. High disinhibitors showed EP augmenting and lows reducing on 3 of the 4 series; differences were significant on two of them. High disinhibitors showed stronger orienting (deceleratory) HR responses to visual and auditory stimuli while lows showed stronger defensive (acceleratory) HR responses. HR responses were significantly correlated across stimulus modalities. Auditory and visual EP slope measures were correlated only for the long ISI series.     

Mediodorsal thalamic lesions impair trace eyeblink conditioning in the rabbit

Rabbits received lesions of the mediodorsal nucleus of the thalamus (MDN) or sham lesions and were subjected to classical eyeblink (EB) and heart rate (HR) conditioning. All animals received trace conditioning, with a.5-sec tone conditioned stimulus, a .5-sec trace period, and a 50-msec periorbital shock unconditioned stimulus. Animals with MDN lesions acquired the EB conditioned response (CR) more slowly than sham-lesioned animals. However, previous studies have shown that MDN damage does not affect delay conditioning using either .5-sec or 1-sec interstimulus intervals. The lesions had no significant effect on the HR CR. These results suggest that information processed by MDN and relayed to the prefrontal cortex is required for somatomotor response selection under nonoptimal learning conditions.     

Auditory streaming: Competition among alternative organizations

It has been suggested that the ON and OFF components of the auditory evoked potential (AEP) may be mediated by independent physiological mechanisms and that the response to a brief tone consists of overlapping ON and OFF responses. Two experiments were performed to evaluate these proposals. First, AEPs were recorded to tonebursts of various durations presented at a fixed rate. As the tonebursts were made longer, they evoked smaller ON responses and larger OFF responses, particularly when either response followed the other by less than 5 sec. This indicates that ON and OFF responses are not physiologically independent. Second, the AEP to a 25-msec tone was compared with the ON response to a 2,000-msec tone. At an interstimulus interval of 4 sec, the difference wave formed by subtracting the latter AEP from the former contained clear N₁ (ca. 100 msec) and P₂ (ca. 180 msec) components, which have been interpreted as an OFF response by some authors. These components in the difference wave were greatly reduced or even inverted at 10-sec interstimulus intervals, however, where interactions between ON and OFF responses to successive stimuli were minimized. This result indicates that any residual OFF response in the AEP to a brief tone is very small in amplitude, if present at all.     

The paraventricular nucleus of the hypothalamus is involved in cardiovascular responses to acute restraint stress in rats

The paraventricular nucleus of the hypothalamus (PVN) has been implicated in several aspects of cardiovascular control. Stimulation of the PVN evokes changes in blood pressure and heart rate. Additionally, this brain area is connected to several limbic structures implicated in behavioral control, as well as to forebrain and brainstem structures involved in cardiovascular control. This evidence indicates that the PVN may modulate cardiovascular correlates of behavioral responses to stressful stimuli. Acute restraint is an unavoidable stressor that evokes marked and sustained cardiovascular changes, which are characterized by elevated mean arterial pressure (MAP) and an intense heart rate (HR) increase. We report on the effect of inhibition of PVN synapses on MAP and HR responses evoked by acute restraint in rats. Bilateral microinjection of the nonspecific synaptic blocker cobalt (CoCl(2), 1 mM/100 nl) into the PVN did not change the HR response or the initial peak of the MAP response to restraint stress, but reduced the area under the curve of the MAP response. Moreover, bilateral microinjection of cobalt in areas surrounding the PVN did not change the cardiovascular response to restraint. These results indicate that synapses in the PVN are involved in the neural pathway that controls blood pressure changes evoked by restraint.     

Decelerative cardiac responsiveness to acoustical stimulation in the near term fetus.

Human fetal cardiac responses (36-39 weeks gestational age) to brief, repeated vocal stimuli (male or female voice uttering the same sentence), given at 90-95 dB SPL ex utero (around 20-30 dB less in utero) during a state of low fetal heart rate (FHR) variability, were examined using highly conservative statistical criteria taking into account each subject's prestimulus FHR variability. Subjects exposed to either of the two stimuli displayed significantly more decelerative heart rate (HR) changes compared to control subjects receiving no stimulation. The decelerative changes started during the first seconds following the onset of stimulation and reached their amplitude peak within 10 or 20 sec, depending on the subject. The direction--HR acceleration or deceleration--and the amplitude of the response depended on prestimulus HR variability only, not on prestimulus level. No major difference was found between the effects of the two voices. The data are compared to previous studies demonstrating fetal decelerative changes to acoustic stimuli of less than 105 dB SPL. The choice of an objective criterion to define an HR response and the possible orienting response nature of the decelerative change are discussed.     

Decelerative cardiac responsiveness to acoustical stimulation in the near term fetus

Human fetal cardiac responses (36-39 weeks gestational age) to brief, repeated vocal stimuli (male or female voice uttering the same sentence), given at 90-95 dB SPL ex utero (around 20-30 dB less in utero) during a state of low fetal heart rate (FHR) variability, were examined using highly conservative statistical criteria taking into account each subject's prestimulus FHR variability. Subjects exposed to either of the two stimuli displayed significantly more decelerative heart rate (HR) changes compared to control subjects receiving no stimulation. The decelerative changes started during the first seconds following the onset of stimulation and reached their amplitude peak within 10 or 20 sec, depending on the subject. The directions—HR acceleration or deceleration—and the amplitude of the response depended on prestimulus HR variability only, not on prestimulus level. No major difference was found between the effects of the two voices. The data are compared to previous studies demonstrating fetal decelerative changes to acoustic stimuli of less than 105 dB SPL. The choice of an objective criterion to define an HR response and the possible orienting response nature of the decelerative change are discussed.     

The effect of temporal distribution in relation to constant frequency of stimuli on habituation of the orienting reaction

The lack of empirical evidence for faster habituation with the regular presentation of stimuli suggests that it is not the constancy of presentation of stimuli, but rather the rate of stimulation--defined as the reciprocal of the harmonic mean of the interstimulus intervals--that determines the speed of habituation. Using alternation of 10-70 and 30-50 sec and a constant sequence of 40 sec, three different rates of stimulation were realized. The hypothesis of faster habituation of skin conductance reactions with higher rates of stimulation was not confirmed. However, a consistent pattern of skin conductance reactions, with an increase in reaction after long intervals and a decrease after short intervals, was found, especially in the group with alternating intervals of 10 and 70 sec. The analysis of an additional group with a random sequence of intervals showed that the response pattern did not result simply from the expired time since last stimulus, i.e., a simple mechanical effect. The possibility of anticipating the stimuli and differing information content of the stimuli are discussed as possible determining factors.     

Neonatal auditory habituation and state change

Groups of newborn babies were exposed to various series of 90 dB SPL noiseband stimuli or to simulated (control) stimuli, while their heart rates (HR) and states of behavioural arousal were monitored. In general the response to stimulation was an accelerative HR change which tended to decrease with repeated stimulation. This seemed to provide evidence of habituation to the auditory stimuli. However, the HR response to sound depended on prestimulus HR and on state, and, when these effects were removed by regression techniques, the progressive decrease in evoked HR with repeated stimulation disappeared, apart from the first few trials. This implied that there was HR habituation to the initial stimuli, but not subsequently, when the apparent HR “habituation” reflected progressive endogenous state changes.     

Variable shock-free times with informative and uniformative stimuli

Three rats were trained on a fixed-ratio escape procedure in which responding produced one of two equiprobable outcomes—a 10-second or 60-second shock-free period. Before the fixed-ratio requirement was satisfied, brief shocks were presented at irregular intervals averaging either 15 or 30 seconds. Two conditions, differing in the relationship between exteroceptive stimuli and the outcomes, were studied. In the uninformative (uncorrelated) condition, the same stimulus was presented during both outcomes. In the informative (correlated) condition, one stimulus was present during the 10-second outcome only; another stimulus was present during the 60-second outcome only. Subjects pressed faster in the uninformative condition than they did in the informative condition. The inadequacy of the information hypothesis in accounting for the findings is discussed, and an alternative hypothesis is offered.     

Heart Rate and Affective Reactions to State Self-Objectification as a Function of Gender

The purpose of the present study was to examine heart rate (HR) and affective reactions to state self-objectification as a function of gender. We examined negative affect, positive affect, guilt, and HR at 6-second and 5-minute intervals across baseline, control, high objectification, low objectification, and cologne conditions in men (n = 53) and women (n = 57). Mixed factorial MANOVA results indicated a statistically significant Gender × Condition interaction. Both men and women showed a cardiac orienting response to high versus low objectification. Cardiac stress reactions to objectification were higher among women. Negative affective reactions to objectification were more pervasive across conditions among women.     

Expectancy and intermittency

When a human subject responds to the second of two closely succeeding stimuli, his reaction time to the second stimulus tends to increase sharply as the interstimulus interval decreases. Controversy has centred on the issue of whether this increase is mainly due to the effects of the first stimulus in producing some kind of block in the central analysing systems or whether it is mainly due to the temporal uncertainty of the second signal, as determined by the distribution of interstimulus intervals used.

By substituting for the first stimulus a spontaneous response on the part of the subject and holding the distribution of interstimulus intervals constant, it is shown that the delays in responding to the succeeding signal are eliminated, even at intervals as short as 50 millisec. This is interpreted as evidence in support of the intermittency hypothesis and as a clear indication that the increase in reaction times normally observed is not a result of the distribution of interstimulus intervals.     

SCORIT: A computer subroutine for scoring electrodermal responses

Given digitized electrodermal response records sampled at a rate of 20/sec and at a sensitivity level between 140 and 170 ohms, a computer subroutine for scoring skin resistance responses is described. It is designed to score multiple responses on a trial, which makes it particularly suitable for classical conditioning experiments involving long interstimulus intervals. The subroutine returns to the main program response latency, base resistance, peak resistance, and time from base to peak. Since SCORIT is written in FORTRAN IV, it can be employed in virtually any modern central computing facility at costs substantially below those involved in hand scoring.     

Infant timekeeping: attention and temporal estimation in 4-month-olds

Four–month–old infants were exposed to sequences in which a 2–s light stimulus alternated with dark interstimulus periods whose length was manipulated to be 3 or 5 s. A predictable on–off pattern occurred for eight trials, but the light stimulus was omitted on the ninth trial. Infants showed heart rate responses on the omission trial that were closely synchronized with the expected recurrence of the stimulus. In addition, these heart rate patterns were observed predominantly in infants who had previously shown high levels of sustained attention in pretests with visual stimuli. These findings indicate remarkable precision in infants' estimation of time intervals, and suggest that the link between time estimation and attentional processes is present in early infancy.     

Immediate reinforcement in delayed reward learning in pigeons

Pigeons were trained on simultaneous red-green discrimination procedures with delayed reward and sequences of stimuli during the delay. In Experiment 1, three stimuli appeared during the 60-second intervals between the correct responses and reward, and the incorrect responses and nonreward. The stimulus that immediately followed a correct response also preceded nonreward, and the stimulus that followed an incorrect response preceded reward. These stimuli were 10 or .33 second in duration for different groups. Stimuli during the remainder of the delay interval differed following correct and incorrect responses. Group 10 initially persisted in the nonrewarded choice, but shifted to a preponderance of rewarded responses after further training. Group .33 rapidly acquired the correct response. Similar results were obtained in Experiment 2 where delay intervals consisted of opposite sequences of two stimuli of equal duration and total delays were 6, 20, or 60 seconds. Early in training, generalization of differential conditioned-reinforcing properties from the conditions preceding reward and nonreward to postchoice conditions had a greater effect relative to backchaining than it did later. It was concluded that delayed-reward learning is best analyzed in terms of the conditioned-reinforcing value of the patterns of cues that follow immediately after rewarded and nonrewarded responses.     

Effects of attending selectively to the spatial position of reflex-eliciting and reflex-modulating stimuli

The question of whether automatic, sensory processes can be modified by selectively directing attention to stimuli was addressed by comparing effects on brainstem reflexes that share a common efferent pathway but have distinct afferent limbs. Subjects judged the duration of brief but intense blink-eliciting tones (Experiment 1) or weak tones preceding a blink-eliciting air puff at interstimulus intervals producing blink inhibition (Experiment 2). Tones occurred unpredictably at left, right, or midline loci; designation of the target location varied across blocks of trials. Latency of blinks to lateralized blink-eliciting targets was facilitated selectively, and the magnitude of blinks evoked by air puff following lateralized prestimulus targets was inhibited selectively. There was no evidence for a midline selective effect. Results appear to support a preset differential processing of stimuli in sensory pathways at low, possibly subcortical, levels and the consequent modification of obligatory, automatic processes.     

The effect of unilateral stellectomy on the regulation of heart rate during behavioral stress

Changes in heart rate were examined during classical aversive conditioning before and after either left (n=10) or right stellectomy (n=8). Heart rate (HR) significantly (p<0.01) increased in response to the conditional stimulus with a further increase noted during the unconditional stimulus. After right stellectomy (RSGx) the aversive stress elicited a significantly (p<0.01) smaller increase in heart rate (peak HR change: control 66.8 +/- 6.0; RSGx 36.1 +/- 6.8 beats/min). In contrast left stellectomy did not significantly alter the heart rate conditional response. Cardioselective beta-adrenergic receptor blockade, metoprolol HCl (BB, 1.0 mg/kg, n=8), significantly (p<0.01) reduced the heart-rate conditional response (peak HR change: control 66.8 +/- 6; BB 25.1 +/- 3.8 beats/min) to the same extent as noted during RSGx. These data suggest that the heart-rate increases elicited by aversive stress are mediated in part by sympathetic nerves that originate or pass through the right stellate ganglion. The residual HR increase noted after RSGx or BB probably results from the withdrawal of cardiac parasympathetic activity.     

The effect of unilateral stellectomy on the regulation of heart rate during behavioral stress.

Changes in heart rate were examined during classical aversive conditioning before and after either left (n = 10) or right stellectomy (n = 8). Heart rate (HR) significantly (p less than 0.01) increased in response to the conditional stimulus with a further increase noted during the unconditional stimulus. After right stellectomy (RSGx) the aversive stress elicited a significantly (p less than 0.01) smaller increase in heart rate (peak HR change: control 66.8 +/- 6.0; RSGx 36.1 +/- 6.8 beats/min). In contrast left stellectomy did not significantly alter the heart rate conditional response. Cardioselective beta-adrenergic receptor blockade, metoprolol HC1 (BB, 1.0 mg/kg, n = 8), significantly (p less than 0.01) reduced the heart-rate conditional response (peak HR change: control 66.8 +/- 6; BB 25.1 +/- 3.8 beats/min) to the same extent as noted during RSGx. These data suggest that the heart-rate increases elicited by aversive stress are mediated in part by sympathetic nerves that originate or pass through the right stellate ganglion. The residual HR increase noted after RSGx or BB probably results from the withdrawal of cardiac parasympathetic activity.     

Concomitant short-term changes in the auditory evoked response, the EEG, and reaction-time performance in relation to the temporal parameters of stimulation

Abstract.— Two experiments are described in which short-term "habituation" of the auditory evoked response (AER) was examined using discrete trains of click stimuli and averaging across trains. In the first study, the stimuli consisted of randomly interspersed trains of regular clicks, at 3 or 10 sec interstimulus interval (ISI). In the second study, the ISIs were also short or long but were irregular varying between 2.4 and 3.6 sec in the former case and 6 to 12 sec in the latter. The N₁-P₂ component of the AER diminished within the 3 sec trains especially for the regular stimulation; no such development occurred with the longer ISI. The total voltage in the background EEG and the reaction-times to the stimuli showed essentially similar changes. The possible mechanisms governing the response decrement are discussed and it is concluded that the data may be interpreted in terms of the Groves and Thompson dual-process theory of habituation.     

A PDP-12 system for on-line acquisition of heart rate data

A research program for investigating the responsiveness of infants at high risk for developing schizophrenia is described briefly. Two measures of reactivity to brief auditory stimuli, the evoked heart-rate response and the evoked potential of the brain, are recorded for analysis by the PDP-12 computer. An interface to the PDP-12, called the R peak detector and R-R interval counter, is used for reducing the EKG to R-R intervals, with an accuracy of ± 1 msec. The current usage of the PDP-12 and its IBM-compatible tape transports is discussed, and proposed developments for on-line statistical analyses of the physiological data and real-time control of the experiment are presented.     

Memory processes in the identification of pitch

A study in pitch identification with three interstimulus intervals is reported. Ss received a pretest, three drills, and a posttest. Results include: (a) superior performance on stimuli requiring only a simple discrimination judgment, (b) inferior performance at longer interstimulus intervals, (c) a greater tendency to repeat correct rather than incorrect responses to adjacent tones at the same pitch level, and (d) a modest, but reliable, improvement in pitch identification accuracy from the pre- to the posttest. Relevance of the results for an interpretation of pitch identification as dependent on memory processes is discussed.