血清差异蛋白质组学在肝癌研究中的进展

随着蛋白质组学相关技术的迅速发展,差异蛋白质组学已成为当今细胞生物学领域中极其活跃的学科.差异蛋白质组学是蛋白质组学的一个分支,研究不同时期、不同条件下蛋白质的表达.差异蛋白质组学的核心技术包括双相电泳、质谱分析.现综述血清差异蛋白质组学在肝细胞癌中的研究进展.     

差异蛋白质组学研究与应用进展

差异蛋白质组学是蛋白质组学的一种重要研究策略,着重于比较不同生物体在不同时刻或状态下蛋白质表达的变化,在目前技术背景下有重要的现实意义。将近年来新发展的差异蛋白组学技术,如激光捕获微切割、蛋白芯片及蛋白质定量研究方面等的技术和差异蛋白组学应用进展进行总结。     

差异蛋白质组学研究与应用进展

差异蛋白质组学是蛋白质组学的一种重要研究策略,着重于比较不同生物体在不同时刻或状态下蛋白质表达的变化,在目前技术背景下有重要的现实意义.将近年来新发展的差异蛋白组学技术,如激光捕获微切割、蛋白芯片及蛋白质定量研究方面等的技术和差异蛋白组学应用进展进行总结.     

蛋白质组学与神经科学:从蛋白质到网络

多蛋白质信号复合物在所有细胞中都很重要,它们在脑内信息处理也有着重要作用。质谱、双向凝胶电泳、蛋白亲和技术、高通量的免疫组化方法和蛋白质组信息学都蛋白质组学研究的主要手段。应用蛋白质组学方法可以研究多蛋白信号复合的功能,多样性与组成的动态变化。因此,蛋白质组学发展使得人们从单个蛋白质的研究上升到对蛋白质网络的研究。蛋白质组学研究将为神经与精神疾病的诊断技术与治疗手段提供一条新的道路。     

兼职蛋白质及其哲学思考

在蛋白质组学天空中,新近提出的兼职蛋白质概念正越来越受到人们的关注。研究发现,某些蛋白质因其细胞定位、细胞类型、寡聚状态的不同及其配体、底物、辅因子和产物在细胞内浓度的变化而表现出不同的功能,从而对细胞和机体产生诸多有益作用,并使其发展出一系列完善机制以实现不同功能间的转换。兼职蛋白质概念的提出不仅改变了一个基因－－一种蛋白质－－一种功能的传统观念,也为大分子间相互作用、细胞复杂性及生物多样性研究     

动物模型蛋白质组学技术在高血压疾病的研究进展

蛋白质组学是研究细胞内全部蛋白质的组成及其活动规律的科学,蛋白质组学研究不但能揭示病因不明疾病的发生和发展机制,并为提供新的诊断技术、治疗方法和药物靶点等有积极的作用。因高血压的病因还不十分清楚,人们希望用分子生物学揭示其神秘的面纱,因此蛋白质组学与高血压的研究,成为高血压模型动物及其临床领域研究的热点,本文对动物模型蛋白质组学技术在高血压疾病的研究进展情况进行综述。     

后基因时代神经源性疼痛研究的焦点：蛋白质网络

后基因时代到来后蛋白质组学技术得到了迅猛发展,系统化和网络化研究神经源性疼痛模型中,神经系统蛋白质结构和功能必将是未来疼痛相关研究的重要组成部分。蛋白质组学方法的广泛应用,将为我们提供一个用于判断疼痛的新工具,更有利于疼痛机理的揭示。     

后基因时代神经源性疼痛研究的焦点:蛋白质网络

后基因时代到来后蛋白质组学技术得到了迅猛发展,系统化和网络化研究神经源性疼痛模型中,神经系统蛋白质结构和功能必将是未来疼痛相关研究的重要组成部分.蛋白质组学方法的广泛应用,将为我们提供一个用于判断疼痛的新工具,更有利于疼痛机理的揭示.     

表面等离子共振技术在蛋白质组学中的应用

被誉为“生命登月计划”的人类基因组计划于2 0 0 1年 2月迎来了它的一个重要里程碑———人类基因组工作框架图完成[1] !它公布了覆盖人类基因组 97%的基因序列 ,并提示仅有 3- 4万个基因编码蛋白质。面对这些浩如烟海的碱基序列 ,如何破译全部遗传信息、解码生命的奥秘成为“后基因组时代”的重要任务。结构基因组学正向功能基因组学、蛋白质组学过渡 ,研究重点变为从整体水平上探讨基因组动态的生物学功能 ,研究蛋白质结构与功能的关系及蛋白质间的相互作用 ,解释人类生命现象何以如此复杂多变。1　 2 1世纪的生命科学是蛋白质组学的时代…     

质荷比为9348Da的肽段对肝母细胞瘤的预后价值

通过蛋白质组学技术中的SELDI-TOF-MS检测肝母细胞瘤的血清生物学标记物,应用ELISA技术检测对比该标记物在正常健康儿童和肝母细胞瘤患儿术前、术后、不同治疗阶段及不同预后患儿不同治疗阶段血清中的含量,结果显示正常组与治愈组中含量最高,术前组、术后组、化疗组、复发组含量较低,且预后良好组多个阶段均明显高于预后不良组,提示质荷比为9 348Da的肽段对肝母细胞瘤的预后及疗效有监测作用,其含量越低,预后越差。     

A COMPARISON BETWEEN THE SEMANTIC DIFFERENTIAL AND SIMILARITY ANALYSIS IN THE MEASUREMENT OF MUSICAL EXPERIENCE

N ordenstreng , K. A comparison between the semantic differential and similarity analysis in the measurement of musical experience. Scand. J. Psychol ., 1968, 9,89–96.—Ten pieces of music were rated by a group of broadcasting sound-controllers using two different scaling techniques, the semantic differential ratings of each sound on 32 scales, and similarity ratings for all pairs of sounds. The two scaling techniques were compared matching factor spaces by transformation analysis. The results indicate almost perfect similarity of the factor structures, which suggests that similarity analysis and the semantic differential in fact measure the same thing.     

Experiences related to expert supervisors' views of ideal therapeutic practice

Aims: This study examines the relationship between expert supervisors' professional experiences and their views about the importance of different psychotherapy techniques. Method: Thirty psychodynamic‐interpersonal (PI) supervisors and 13 cognitive‐behavioural (CB) supervisors (N=43) were instructed to rate 20 therapeutic techniques according to how characteristic each technique is of an ideally‐conducted session within their theoretical orientation. The measure used for this assessment was the Comparative Psychotherapy Process Scale. Results: Findings demonstrated that supervisors' beliefs about ideal therapeutic techniques are related to their number of years in post‐graduate clinical practice, training, and supervising trainees, as well as to the number of publications they have authored. Further, experience within a given orientation tends to lead to a greater endorsement of techniques within that orientation, although this finding is more uniform for CB supervisors than PI supervisors. Finally, differential types of experience lead to differential focusing on specific techniques. Discussion: The clinical importance of these findings is explored.     

A Review of Diagnostic Techniques in the Differential Diagnosis of Epileptic and Nonepileptic Seizures

The diagnosis of psychogenic nonepileptic seizures (PNES) is complex. Long-term electroencephalogram monitoring with video recording (video EEG) is the most common method of differential diagnosis of epilepsy and PNES. However, video EEG is complex, costly, and unavailable in some areas. Thus, alternative diagnostic techniques have been studied in the search for a diagnostic method that is as accurate as video EEG, but more cost effective, convenient, and readily available. This paper reviews the literature regarding possible diagnostic alternatives and organizes findings into 7 areas of study: demographic and medical history variables, seizure semiology, provocative testing, prolactin levels, single photon emission computed tomography, psychological testing, and neuropsychological testing. For each area, the literature is summarized, and conclusions about the accuracy of the technique as a diagnostic tool are drawn. Overall, it appears unlikely that any of the reviewed alternative techniques will replace video EEG monitoring; rather they may be more successful as complementary diagnostic tools. An important focus for further investigations involves combinations of diagnostic techniques for the differential diagnosis of epilepsy and PNES.     

La chaise vide en psychothérapie : origines et développements

Working with the empty chair or the two-chair dialogue techniques is used in experiential techniques. The latter are practiced in various psychotherapy forms. The present article demonstrates the origins of these techniques in the Gestalt and Person-centered models of psychotherapy, as well as presents their current developments in experiential psychotherapy approaches. The techniques presented are classified according to their objectives, namely: clarification and meaning construction, and modification of internal determinants of the presenting problem. The following techniques are briefly presented, illustrated with clinical examples and discussed: treatment of internal conflict split; treatment of self-interruptive process; unfinished business dialogue; self-soothing dialogue; cognitive elements in the one-person role-play; affective elements in the one-person role-play. We discuss the empirical evidence of these techniques, by referring to efficacy and effectiveness studies, as well as process studies, and conclude with a reflection on the differential indication of the techniques, in the context of novel clinical and research perspectives.     

Promoting response variability and stimulus generalization in martial arts training

The effects of reinforcement and extinction on response variability and stimulus generalization in the punching and kicking techniques of 2 martial arts students were evaluated across drill and sparring conditions. During both conditions, the students were asked to demonstrate different techniques in response to an instructor's punching attack. During baseline, the students received no feedback on their responses in either condition. During the intervention phase, the students received differential reinforcement in the form of instructor feedback for each different punching or kicking technique they performed during a session of the drill condition, but no reinforcement was provided for techniques in the sparring condition. Results showed that both students increased the number of different techniques they performed when reinforcement and extinction procedures were conducted during the drill condition, and that this increase in response variability generalized to the sparring condition.     

肺功能检查对婴幼儿哮喘诊断的价值

如何对婴幼儿哮喘进行早期诊断和进行早期干预已成为目前儿童哮喘防治的主要话题。随着肺功能检测技术的进步,临床上已有多项肺功能检测技术可应用于婴幼儿。正确地理解和掌握这些检测技术可为婴幼儿哮喘的诊断和鉴别诊断提供重要依据。本文对这些不同检测技术的原理、参数及其临床意义进行了阐述。     

Adverse effects of differential parental attention

In two independent parent training projects (Kansas and Mississippi), mothers of deviant young children were observed to follow almost all child behaviors with attention. The mothers were then trained to use differential attention procedures to increase their child's appropriate behaviors and to decrease deviant behaviors. Contrary to expectations, the differential attention procedure produced substantial increases in deviant behavior for four of the children. This adverse effect was maintained over many sessions and was replicated in single organism, reversal designs. A fifth child showed no change. A sixth child showed some improvement. However, this effect was not recovered in a second application of differential attention, and the child became worse. The results underline the importance of subject generality in applied behavior analysis and strongly suggest that service programs using operant techniques must carefully evaluate their effects on behavior.     

Consensus Rorschachs and Related Procedures for Studying Interpersonal Patterns

A psychological "type" characterized by repetitive and compulsive action tendencies but not by obsessive thoughts was delineated. It was shown how this syndrome could be identified through differential inter-test patterning on projective techniques. The possibility of improving psychodiagnostic accuracy by stipulating expected configurations among tests in a battery was noted.