Automated Measurement of Mouse Freezing Behavior and its Use for Quantitative Trait Locus Analysis of Contextual Fear Conditioning in (BALB/cJ × C57BL/6J)F2 Mice |
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| Authors: | Ver nica S. Valentinuzzi, Daniel E. Kolker, Martha Hotz Vitaterna, Kazuhiro Shimomura, Andrew Whiteley, Sharon Low-Zeddies, Fred W. Turek, Elenice A.M. Ferrari, Richard Paylor, Joseph S. Takahashi |
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| Affiliation: | Verónica S. Valentinuzzi, Daniel E. Kolker, Martha Hotz Vitaterna, Kazuhiro Shimomura, Andrew Whiteley, Sharon Low-Zeddies, Fred W. Turek, Elenice A.M. Ferrari, Richard Paylor, and Joseph S. Takahashi |
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| Abstract: | The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ ×C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test. |
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