HMGA1及MMP2在非小细胞肺癌中的表达、临床意义

采用免疫组织化学方法检测高迁移率族蛋白1(HMGA 1)及基质金属蛋白酶2(MMP2)在非小细胞肺癌(NSCLC)中的表达.结果显示在NSCLC中HMGA1及MM P2的阳性表达率(57.1％、63.5％)显著高于正常肺组织,其阳性表达与性别、年龄无关(P＞0.05),与肺癌分化程度、TNM分期和有无淋巴结转移密切相关(P＜0 05).因此临床上,联合检测HMGA 1及MM P2蛋白对NSCLC的诊断及判断其预后可能具有一定的参考价值.     

糖尿病高血压和糖尿病肾病患者血清MMP-2及TIMP-1的表达及意义

为了探讨MMP-2、TIMP-1在DMEH和DN发病中的意义,随机收集T2DM患者146例,其中SDM 57例,DMEH 38例,DN 51例,健康对照38例。用ELISA法测定MMP-2、TIMP-1的含量。结果显示,DM患者尤其是DMEH和DN患者血清MMP-2明显低于健康对照组,P均〈0.01,且随病情发展呈逐渐降低趋势。而血清TIMP-1随病情的发展则呈现明显的上升趋势,SDM、DMEH组和DN组与健康对照组比较,分别为P〈0.05,P〈0.01和P〈0.01。因此,MMP-2和TIMP-1可能参与了DMEH和DN的发生发展过程,定期检测DM患者血清MMP-2和TIMP-1的含量变化有助于DM并发症的早期诊断和疗效观察。     

糖尿病高血压和糖尿病肾病患者血清MMP-2及TIMP-1的表达及意义

为了探讨MMP-2、TIMP-1在DMEH和DN发病中的意义,随机收集T2DM患者146例,其中SDM 57例,DMEH 38例,DN 51例,健康对照38例。用ELISA法测定MMP-2、TIMP-1的含量。结果显示,DM患者尤其是DMEH和DN患者血清MMP-2明显低于健康对照组,P均〈0.01,且随病情发展呈逐渐降低趋势。而血清TIMP-1随病情的发展则呈现明显的上升趋势,SDM、DMEH组和DN组与健康对照组比较,分别为P〈0.05,P〈0.01和P〈0.01。因此,MMP-2和TIMP-1可能参与了DMEH和DN的发生发展过程,定期检测DM患者血清MMP-2和TIMP-1的含量变化有助于DM并发症的早期诊断和疗效观察。     

Caspase-3和c-IAP1在胃癌组织中的表达及意义

探讨胃癌组织中Caspase-3和c-IAP1的表达水平及意义。通过蛋白印迹Western blot和实时荧光定量PCR技术检测50例胃癌组织和40例正常胃黏膜组织中Caspase-3和c-IAP1的蛋白和基因mRNA的表达水平。结果与正常胃黏膜组织相比,胃癌组织中Caspase-3蛋白和mRNA的表达水平显著降低(P0.05),Caspase-3蛋白和mRNA的低表达与胃癌的组织分化程度、TNM临床分期、淋巴结转移有关(P0.05),而与性别、年龄及浸润深度无关(P0.05);与正常胃黏膜组织相比,胃癌组织中c-IAP1蛋白和mRNA的表达水平显著升高(P0.05),c-IAP1蛋白和mRNA的高表达与胃癌的组织分化程度、TNM临床分期、淋巴结转移及浸润深度有关(P0.05)),而与性别、年龄无关(P0.05)。Caspase-3和c-IAP1在胃癌中的异常表达均与胃癌的发生、发展有关。     

IGFBP-2及IGFBP-6在结直肠癌中的表达及意义

研究胰岛素样生长因子结合蛋白2（IGFBP-2）及胰岛素样生长因子结合蛋白6（IGFBP-6）在结直肠癌中的表达及临床意义。采用免疫组织化学法及RT-PCR方法检测结直肠癌、结直肠腺瘤及癌旁正常黏膜组织中IGFBP-2及IGFBP-6的蛋白及mRNA的表达情况,结合临床病理资料进行统计学分析。IGFBP-2及IGFBP-6蛋白的阳性表达及mRNA的表达量在三组组织中均存在异常表达情况,且差异有明显的统计学意义（P〈0.05）,在结直肠癌组IGFBP-2、IGFBP-6的阳性表达在肿瘤浸润深度、有无淋巴结转移及Duke＇s分期存在差异,有明显的统计学差异（P〈0.05）。临床可以把二者作为结直肠癌诊断及预后的早期预测指标。     

胰岛素样生长因子结合蛋白3mRNA在肾癌中的表达

为探讨胰岛素样生长因子结合蛋白3(IGFBP-3)mRNA在肾癌(RCC)中的表达,采用半定量RT-PCR方法进行检测,检测56例RCC组织标本及56例癌旁组织标本中IGFBP-3的表达.结果显示RCC与癌旁组织之间IGFBP-3表达差异有显著性(P＜0.05),IGFBP-3在透明细胞癌的强阳性表达,高于其他病理类型(P均＜0.05),并与病理分级相关.因此,IGFBP-3在RCC组织中的表达具有显著性,其在肾透明细胞癌中的强阳性表达具有特异性.     

乳腺癌中ING4和MMP-9相关性及临床应用价值

研究乳腺癌中生长抑制因子4(ING4)和基质金属蛋白酶-9(MMP-9)相关性及意义,采用组织芯片免疫组化技术检测83例乳腺癌组织、16例癌旁组织中ING4、MMP-9的表达,阳性表达率分别是56.6%、87.5%和71.1%、37.5%,与组织学分级、淋巴结转移、临床分期有关(P0.05)。两者表达呈负相关,提示ING4和MMP-9在乳腺癌的发生、发展过程中起重要调控作用,临床联合检测可为乳腺癌早期诊断、临床个性化治疗及预后提供一定参考。     

CyclinD1、VEGF、Flt-1在肝细胞癌中的表达及临床意义

探讨肝细胞癌( Hepatocellular carcinoma,HCC)、胆管癌、肝硬化及正常肝组织中周期素D1 (CyclinD1)、血管内皮生长因子(VEGF)及血管内皮生长因子受体(Flt-1)蛋白表达的意义及其与肝癌生物学行为关系.对52例符合要求的HCC组织、10例胆管癌组织、10例肝硬化组织及10例正常肝组织采用免疫组化S-P法检测CyclinD1、VEGF及Flt-1蛋白.CyclinD1、VEGF及Flt-1在HCC的强阳性表达率分别为55.8％、55.8％及67.3％,均显著高于胆管癌、肝硬化及正常肝组织(P＜0.001).CyclinD1及VEGF表达与肿瘤包膜完整、血管侵犯及肿瘤分化程度明显有关(P＜0.05);Flt -1与肿瘤分化程度明显有关(P＜0.05).CyclinD1与VEGF间、CyclinD1与Flt-1间及VEGF与Flt-1间的蛋白表达均呈正相关(R=0.445,P＜0.01、R=0.368,P＜0.01、R=0.332,P＜0.01).CyclinD1、VEGF及Flt-1蛋白的过表达可促使肝癌细胞增殖,使肝癌细胞具有更强的侵袭力,与肝癌的发生、发展密切相关.     

金属蛋白酶-9(MMP-9)及其组织抑制物-1(TIMP-1)在胰腺癌中的表达

探讨MMP-9和TIMP-1在胰腺癌癌细胞及间质的表达与胰腺癌分化程度、分期之间的关系.采用免疫组织化学方法,检测MMP-9和TIMP-1在胰腺癌细胞及间质细胞的表达.MMP-9在胰腺癌细胞和间质组织中均有表达.TlMP-1主要表达在胰腺癌细胞中.MMP-9在胰腺癌间质细胞中的表达高于胰腺癌癌旁组织间质细胞,与胰腺癌的病理分级、分期呈正相关.结论认为MMP-9在胰腺癌间质细胞的表达可能参与胰腺癌的浸润和转移,对预后判断、诊断及治疗具有一定的意义.     

APC及p63在人口腔鳞状细胞癌发生中的表达及病理意义

为了探讨抑癌基因APC及p63在口腔鳞状细胞癌中的蛋白表达水平和病理意义,实验收集46例人口腔鳞癌石蜡包埋组织,采用elivision染色法检测口腔正常黏膜上皮、上皮异常增生和口腔鳞癌组织中的APC及p63的表达变化,最后将各组份的免疫组化结果与各病例的临床病理学资料使用Kruskal- Wallis检验或Spearman相关性检验,进行统计学处理.结果显示APC蛋白胞浆表达水平与口腔鳞癌的分化呈正相关(P＜0.05),p63蛋白胞核表达水平与口腔鳞癌的分化呈负相关(P＜0.05).APC与其淋巴结有否转移无相关性,而p63与其淋巴结转移有否呈负相关(P＜0.05).因此,APC及p63的表达与口腔鳞癌的发生密切相关,而且与口腔鳞癌的分化程度有关,提示APC和p63可作为口腔鳞癌的发生和预后的检测指标.     

Ab initio investigation of electron-phonon interaction in LaSn3 and CaSn3

ABSTRACT

We present results for the structural, electronic, vibrational, and electron-phonon coupling properties of LaSn₃ and CaSn₃ adopting the simple cubic AuCu₃-type structure obtained using the the generalised gradient approximation of the density functional theory and plane wave ab initio pseudopotential method. Our electronic results show that both materials display metallic character with several bands, which have mainly Sn 5p character, crossing the Fermi level. The calculated phonon spectrum of LaSn₃ accords very well with reported experimental measurements. The weights of the peaks in the Eliashberg spectral function of both compounds are enhanced with the use of experimental lattice constant in our electron-phonon calculation, increasing the value of average electron phonon coupling parameter from 0.876 to 0.937 for LaSn₃ (by 7%) and from 0.642 to 0.725 for CaSn₃ (by 13%). The use of experimental lattice constant also improves the agreement between theoretical and experimental values of the superconducting temperature for both compounds.     

Comparison of the RT3 Research Tracker and Tritrac R3D accelerometers

This study compared the RT3 Research Tracker accelerometer to the Tritrac R3D accelerometer in both laboratory and field settings and tested the hypothesis that the RT3 records higher physical activity counts and smaller standard deviations than the R3D. The RT3 is relatively new and untested and its concurrent validity with existing instruments and physical activity needs to be assessed before being used in research. In this study the RT3 had higher average recordings of physical activity counts in all of the nine testing situations than the R3D. However, in terms of agreement between the instruments, the RT3 might be 582 below or 1,236 above (activity counts) the R3D in assessing physical activity. These results do not establish that the RT3 is more consistently measuring higher physical activity counts than the R3D. Comparing vector magnitude with oxygen consumption and heart rate across the 0% grade testing conditions indicated that the RT3 and R3D are sensitive to changes in various intensities of level ambulation. When the 5%, 10%, and 15% grade on the treadmill protocols were analyzed, low correlations between oxygen consumption and heart rate with vector magnitude responses were found for both the RT3 and R3D. Differences in agreement between the RT3 and R3D did not vary in any systematic way over the range in testing conditions which substantiates that the RT3 and R3D accelerometers are sensitive on flat surfaces but are insensitive to changes in grade.     

Discrimination of 3-D shape and 3-D curvature from motion in active vision

We examined the ability of human observers to discriminate between different 3-D quadratic surfaces defined by motion, and with head position fed back to the stimulus to provide an up-to-date dynamical perspective view. We tested whether 3-D shape or 3-D curvature would affect discrimination performance. It appeared that discrimination of 3-D quadratic shape clearly depended on shape but not on the amount of curvature. Even when the amount of curvature was randomized, subjects’ performance was not altered. On the other hand, the discrimination of 3-D curvature clearly depended linearly on curvature with Weber fractions of 20% on the average and, to a small degree, on 3-D shape. The experiment shows that observers can easily separate 3-D shape and 3-D curvature, and that Koenderink’s shape index and curvedness provide a convenient way to specify shape. These results warn us against using just any arbitrary 3-D shape in 3-D shape perception tasks and indicate, for example, that emphasizing 3-D shape in computer displays by exaggerating curvature does not have any effect.     

Informational Realism and World 3

This paper takes up a suggestion made by Floridi that the digital revolution is bringing about a profound change in our metaphysics. The paper aims to bring some older views from philosophy of mathematics to bear on this problem. The older views are concerned principally with mathematical realism—that is the claim that mathematical entities such as numbers exist. The new context for the discussion is informational realism, where the problem shifts to the question of the reality of information. Mathematical realism is perhaps a special case of informational realism. The older views concerned with mathematical realism are the various theories of World 3. The concept of World 3 was introduced by Frege, whose position was close to Plato’s original views. Popper developed the theory of World 3 in a different direction which is characterised as ‘constructive Platonism’. But how is World 3 constructed? This is explored by means of two analogies: the analogy with money, and the analogy with meaning, as explicated by the later Wittgenstein. This leads to the development of an account of informational realism as constructive Aristoteliansim. Finally, this version of informational realism is compared with the informational structural realism which Floridi develops in his 2008 and 2009 papers in Synthese. Similarities and differences between the two positions are noted.     

Crystal growth and defect study of BiSbTe3

BiSbTe₃ single crystals have been grown by the Bridgman technique. Microscopic observations of the as-grown crystals reveal typical features, such as striations on the top free surface, which are attributed to the effect of growth conditions. A nitric-acid-based reagent capable of revealing dislocations has been developed and tested. Etch pits are produced at the dislocation sites, but some discrepancies have been observed on matched cleavage surfaces. The structural difference between the matched cleavage surfaces is discussed.     

Structural,elastic, electronic,phonon and thermal properties of Ir3Ta and Rh3Ta alloys

We have studied the structural, elastic, electronic, phonon and thermodynamic properties of Ir₃Ta and Rh₃Ta alloys, using ab initio calculations. For the L1₂ phase, we report the calculated lattice constants, bulk modulus and elastic constants, and these values are compared with previously published values. We also derive the elastic constants from the values of the slopes of the acoustic branches in the phonon dispersion curves. The band structures show that both materials are metallic. The phonon dispersion curves, and their corresponding total and projected densities of states, are obtained using a linear response in the framework of the density functional perturbation theory. The specific heat capacity at constant volume and different temperatures is calculated, and this aspect is discussed using the quasi-harmonic approximation.